AHK tripeptide-3, also known as L-Alanyl-L-Histidyl-Lysine, is a human-body homologous biomimetic linear tripeptide developed with mature peptide synthesis technology. As a core signal peptide and metal-binding peptide, it features a small molecular structure with no disulfide bonds, and can chelate Cu²⁺ to form the highly active AHK-Cu complex. This product is widely recognized as a high-performance cosmetic raw material and research-grade peptide, covering application scenarios from anti-hair loss hair growth, skin anti-aging repair to tissue regeneration related scientific studies.
Kpeptide have Reliable Supply & Service, Multiple available specifications from mg to kg level, meeting both lab research and industrial production demands, Professional technical team provides formulation guidance and application solution support.
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AHK Tripeptide-3 COA
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| Certificate of Analysis | ||
| Compound name | AHK Tripeptide-3 | |
| Grade | Pharmaceutical grade | |
| CAS No. | 126828-32-8 | |
| Quantity | 15g | |
| Packaging standard | PE bag+Al foil bag | |
| Manufacturer | Shaanxi BLOOM TECH Co., Ltd | |
| Lot No. | '202601090056 | |
| MFG | Jan 9th 2026 | |
| EXP | Jan 8th 2029 | |
| Structure |
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| Item | Enterprise standard | Analysis result |
| Appearance | White or almost white powder | Conformed |
| Water content | ≤5.0% | 0.32% |
| Loss on drying | ≤1.0% | 0.28% |
| Heavy Metals | Pb≤0.5ppm | N.D. |
| As≤0.5ppm | N.D. | |
| Hg≤0.5ppm | N.D. | |
| Cd≤0.5ppm | N.D. | |
| Purity (HPLC) | ≥99.0% | 99.98% |
| Single impurity | <0.8% | 0.26% |
| Total microbial count | ≤750cfu/g | 450 |
| E. Coli | ≤2MPN/g | N.D. |
| Salmonella | N.D. | N.D. |
| Ethanol (by GC) | ≤5000ppm | 500ppm |
| Storage | Store in a sealed, dark, and dry place below -20°C | |
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Research Applications in Hair Follicle Biology and Alopecia Mechanism Research
As a highly spcific, follicle-targeted bioactive peptide independently optimized and standardized by Kpeptide, AHK Tripeptide-3 (Alanyl-Histidyl-Lysine) has become a core research reagent and functional active substance widely adopted in contemporary hair biology, trichology and anti-alopecia pharmacological research. Different from conventional broad-spectrum cosmetic peptides, AHK Tripeptide-3 features precise molecular targeting performance and excellent biological safety, which enables it to serve as a standard experimental material for exploring the physiological regulation mechanism of hair follicles, pathological deterioration mechanism of alopecia, and the efficacy verification of new hair-care active ingredients.
In basic scientific research, AHK Tripeptide-3 is extensively applied in in vitro human dermal papilla cell (DPC) culture models and ex vivo human hair follicle organ explant culture systems. Dermal papilla cells are recognized as the core regulatory hub of hair follicle growth, cycle transformation and follicle vitality maintenance. Researchers utilize high-purity Kpeptide AHK Tripeptide-3 to intervene in DPCs under normal culture conditions and oxidative stress-induced damaged conditions,
so as to systematically explore the regulatory mechanism of peptide molecules on dermal papilla cell proliferation, viability maintenance, senescence inhibition and apoptotic signal pathway regulation. It can be used for quantitative detection of key apoptotic biomarkers including Caspase-3, PARP, Bcl-2 and Bax, helping researchers clarify the protective effect of active peptides on hair follicle progenitor cells and the molecular mechanism of inhibiting follicle cell apoptosis. In the research of hair follicle cycle regulation and microenvironmental improvement, Kpeptide AHK Tripeptide-3 acts as a key research tool to study the VEGF-mediated dermal microvascular angiogenesis pathway and the TGF-β1-induced hair follicle miniaturization pathway. It supports in-depth exploration of how bioactive substances reverse follicle atrophy, block the transformation from anagen to catagen, and prolong the growth cycle of hair follicles. This application covers the core pathological research directions of common clinical alopecia types, including androgenetic alopecia, stress-induced telogen effluvium and nutritional deficiency alopecia,
and provides reliable experimental support for revealing the pathogenesis of different types of alopecia. In addition, AHK tripeptide-3 is widely used in high-throughput active ingredient screening and formula synergistic mechanism verification in the field of hair care research. R&D institutions adopt this standardized peptide raw material to evaluate the synergistic anti-alopecia efficacy of compound formulas, explore the optimal concentration window of peptide intervention, and verify the compatibility effect with plant extracts, vitamins and other hair-care actives. Benefiting from Kpeptide's strict purification technology and batch stability control, the product eliminates experimental errors caused by unstable raw material activity and impurity interference, greatly improving the repeatability and credibility of hair follicle mechanism research and efficacy screening experiments.
Comprehensive Research Progress of AHK Tripeptide in Hair Academic and Industrial Research
Foundational Theoretical Research Stage
The basic research system of AHK Tripeptide-3 in hair biology was initially established and verified by classic academic research (Pyo et al., 2007), which laid an irreplaceable theoretical foundation for the subsequent industrialization and in-depth research of AHK peptide. This landmark study systematically confirmed the specific regulatory effect of AHK-Cu complex (the active chelated form of AHK Tripeptide-3) on human hair follicle cells and tissues. The experimental results clearly proved that AHK-series peptides can significantly promote the proliferation activity of human dermal papilla cells in vitro, effectively up-regulate the expression level of vascular endothelial growth factor (VEGF), improve microvascular blood perfusion around hair follicles, and provide sufficient nutritional support for follicle growth.
Meanwhile, the study verified that AHK Tripeptide-3 can significantly inhibit the expression of TGF-β1, a key factor leading to hair follicle miniaturization and alopecia. By regulating the Bcl-2/Bax protein ratio, it effectively reduces the expression of pro-apoptotic markers such as Caspase-3 and PARP,
inhibits oxidative stress-induced damage and apoptosis of dermal papilla cells, and maintains the vitality of hair follicle progenitor cells. In ex vivo human hair follicle explant experiments, AHK tripeptide-3 was proven to significantly promote hair shaft elongation, prolong the anagen phase of hair follicles, and delay follicle senescence and atrophy. Subsequent academic reviews and trichology research monographs have officially classified Kpeptide AHK Tripeptide-3 as a follicle-targeted specific active peptide, forming a clear technical differentiation from traditional broad-spectrum GHK-series copper peptides in mechanism and efficacy, and further consolidating the unique research value of AHK peptides in professional hair regrowth fields.
In Vitro & Ex Vivo Model Verification Stage (Mature & Industrialized)
After years of iterative verification by global academic institutions and cosmetic R&D teams, the research and application of AHK Tripeptide-3 in hair follicle research has entered a mature and systematic verification stage. At present,
the mainstream research system is dominated by standardized in vitro cell models and ex vivo isolated human hair follicle organ culture models, with complete and standardized experimental verification systems formed. A large number of repeated experimental data have confirmed the stable efficacy of AHK Tripeptide-3 in activating dermal papilla cells, regulating hair follicle cycle, resisting follicle oxidative damage and improving scalp microcirculation.
As a professional peptide brand focusing on bioactive peptide optimization and production, Kpeptide further optimizes the molecular activity and batch stability of AHK Tripeptide-3 on the basis of traditional academic research. Through independent low-temperature synthesis and high-purity purification technology, Kpeptide solves the problems of low purity, unstable activity and poor repeatability of traditional AHK peptide raw materials, providing standardized, research-grade peptide raw materials for global hair follicle mechanism research, new hair-care product development and efficacy verification. At this stage, the cell-level and tissue-level efficacy and mechanism of AHK Tripeptide-3 have been fully verified, and it has become a mature and mainstream research tool and cosmetic active raw material in the global trichology research field.
Current Frontier Research & Ongoing Exploration Directions
With the continuous upgrading of cosmetic peptide technology and in-depth exploration of hair loss pathogenesis, the research of AHK Tripeptide-3 has expanded to multiple frontier directions in recent years. First, scholars and industrial R&D teams are committed to exploring efficient delivery system optimization, modifying AHK Tripeptide-3 through lipid encapsulation, nanocarrier loading and molecular lipophilic modification, to solve the problem of limited scalp transdermal absorption of traditional peptides and further improve the bioavailability of follicle-targeted intervention. Second, the synergistic compound efficacy research of AHK Tripeptide-3 is in full swing. Researchers combine AHK Tripeptide-3 with anti-oxidant ingredients, anti-androgen alopecia actives and scalp repair components to explore multi-pathway synergistic hair-care mechanisms, achieve complementary efficacy, and develop high-efficiency compound anti-alopecia formulas. Third, in-depth exploration of concentration-effect relationship and biosafety window is carried out to clarify the optimal effective concentration,
safe tolerance range and dose-dependent efficacy difference of AHK Tripeptide-3, providing accurate data support for industrial formula design. In addition, the industry is also conducting in-depth comparative research on free AHK tripeptide-3 and copper-chelated AHK-Cu complex, distinguishing the activity differences, action pathways and application scenarios of the two forms, so as to realize precise application in different hair care formulas and scientific research experiments.
Overall Industry Research Status & Kpeptide's Continuous Technical Iteration
At present, the global academic and industrial research of AHK Tripeptide-3 has formed a complete system from basic mechanism exploration to mature application verification. The core mechanism and cell/tissue-level efficacy have been fully demonstrated and widely recognized, while high-standard human clinical efficacy research is still in the progressive exploratory stage, which is also the key research direction of the next stage of trichology. As a leading professional peptide brand, Kpeptide has long-term layout in the innovative research of AHK Tripeptide-3. On the basis of mature basic research,
the brand continues to carry out internal technical iteration, including molecular structure optimization, purification process upgrading, activity stability improvement and anti-interference performance optimization of raw materials.
Kpeptide persists in combining academic frontier research with industrial practical application, continuously polishing product performance, and providing high-purity, high-stability, batch-consistent research-grade and cosmetic-grade AHK Tripeptide-3 raw materials for global universities, scientific research institutions and high-end cosmetic R&D enterprises. It effectively supports the continuous innovation and technological upgrading of global hair follicle biology research and high-efficiency anti-alopecia product development.
Production process
Raw Material Screening & Preparation: High-purity amino acid monomers and resin carriers are strictly screened and pretreated to remove impurities, moisture and residual contaminants, laying a pure foundation for accurate peptide chain synthesis.
Solid-Phase Peptide Synthesis: Precise amino acid coupling reaction is carried out in strict accordance with the AHK Tripeptide-3 sequence (Alanine-Histidine-Lysine) through automated synthesis equipment to complete orderly peptide chain assembly.
Resin Cleavage & Crude Peptide Separation: The synthesized peptide chain is cleaved from the solid resin carrier by professional cleavage solution, and the crude AHK tripeptide is separated and precipitated to remove redundant protective groups and resin residues.
High-Precision HPLC Purification: Preparative high-performance liquid chromatography is applied for gradient purification to isolate target peptide components, effectively eliminate heteropeptides, by-products and residual impurities, greatly improving product purity.
Sterile Filtration & Depyrogenation: The purified peptide solution undergoes sterile ultrafiltration and depyrogenation treatment to remove bacteria, heat sources and macromolecular impurities, meeting cosmetic and research-grade safety standards.
Low-Temperature Lyophilization: Adopting vacuum low-temperature freeze-drying technology to remove moisture gently without damaging peptide activity, forming loose and stable AHK Tripeptide-3 powder.
Transportation of Kpeptide AHK Tripeptide-3
Kpeptide AHK Tripeptide-3 is a bioactive peptide sensitive to light, moisture and extreme temperature fluctuations, so the whole transportation workflow is designed to maintain its chemical integrity and biological activity.
After final quality inspection, the peptide product is sealed in food-grade inert primary containers, protected with desiccants to block moisture ingress. Primary containers are then placed into light-shielding, shock-resistant secondary packaging with cushioning materials to avoid mechanical damage during handling.
Each shipment is attached with complete documents including COA, MSDS and packing list. The cargo is transported under controlled ambient temperature conditions, away from direct sunlight and freezing or overheating environments. Real-time tracking is available for each consignment to monitor transit status.
Upon arrival, the packaging integrity will be checked first before storage. This standardized logistics protocol minimizes activity loss and ensures batch consistency of Kpeptide AHK Tripeptide-3 delivered to customers.
References
1. AHK-CU VS GHK-CU: THE DIFFERENCE BETWEEN GHK-CU AND AHK-CU.
2.Pickart, L., & Margolina, A. (2018). Glycyl-L-histidyl-L-lysine and its copper complex: Biological activities and molecular mechanisms. Journal of Biomaterials Science, Polymer Edition, 29(14), 1720–1742.
3.Badenhorst, T., Svirskis, D., & Merrilees, M. (2020). Peptide delivery strategies for skin and hair regenerative cosmetics. Journal of Cosmetic Dermatology, 19(11), 2801–2814.
4.Magerl, M., Kloepper, J. E., & Paus, R. (2010). Human hair follicle organ culture: A versatile ex vivo model for hair research. Experimental Dermatology, 19(10), 865–874.
5.Chan, W. C., & White, P. D. (2000). Fmoc Solid Phase Peptide Synthesis: A Practical Approach. Oxford University Press.
6.Pyo, H. K., et al. (2007). The effect of tripeptide-copper complex on human hair growth in vitro. Archives of Pharmacal Research, 30(7), 834–839.
7.Pickart, L., & Margolina, A. (2018). Glycyl-L-histidyl-L-lysine and its copper complex: Biological activities and molecular mechanisms. Journal of Biomaterials Science, Polymer Edition.
8.Magerl, M., et al. (2010). Human hair follicle organ culture: A versatile ex vivo model for hair research. Experimental Dermatology.
9.Chan, W. C., & White, P. D. (2000). Fmoc Solid Phase Peptide Synthesis: A Practical Approach. Oxford University Press.
10.Badenhorst, T., et al. (2020). Peptide delivery strategies for skin and hair regenerative cosmetics. Journal of Cosmetic Dermatology.
Frequently Asked Questions
Q: What makes Kpeptide AHK Tripeptide-3 different from ordinary AHK peptides on the market?
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A: Kpeptide AHK Tripeptide-3 is independently optimized and mass-produced by our professional peptide laboratory. Compared with generic AHK peptides, it features higher monomer purity, better batch consistency, and stable biological activity. We adopt exclusive low-temperature synthesis and high-purity HPLC purification technology to eliminate invalid heteropeptides and impurities, ensuring stronger follicle-targeting efficacy and better formulation compatibility. It is a premium-grade peptide specifically polished for hair regrowth and scalp anti-aging applications.
Q: What are the core efficacy and applicable scenarios of Kpeptide AHK Tripeptide-3?
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A: This tripeptide focuses on hair follicle activation and scalp microenvironment repair. It effectively promotes dermal papilla cell proliferation, accelerates scalp microcirculation, prolongs the hair anagen phase, and relieves thinning hair and stress hair loss. Meanwhile, it boosts collagen synthesis for anti-aging and skin repair. It is widely suitable for hair regrowth serums, scalp repair essences, anti-hair loss tonics, and high-end anti-aging skincare products.
Q: Does your AHK Tripeptide-3 support scientific research and efficacy verification?
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A: Absolutely. Kpeptide AHK Tripeptide-3 fully matches international classic research standards. It can be directly applied in dermal papilla cell experiments, hair follicle explant culture, hair loss mechanism research, and efficacy screening. We provide stable research-grade raw materials and complete experimental data support, helping customers complete product efficacy verification, academic research and brand technical endorsement.
Q: Is Kpeptide AHK Tripeptide-3 safe for sensitive scalp and skin?
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A: Yes. The product is non-toxic, non-irritating and non-allergenic after strict safety evaluation. It removes residual toxic reagents and heat sources through professional depyrogenation and sterile filtration processes. With mild and efficient characteristics, it is friendly to sensitive scalp and fragile facial skin, suitable for high-end mild and safe cosmetic formula development.
Q: Can AHK Tripeptide-3 be used alone or compounded with other actives?
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A: It can be used alone for basic scalp repair and anti-hair loss efficacy. It also presents excellent synergistic effects when compounded with copper ions, growth factors, plant extracts and anti-aging peptides. Compounded formulas can achieve multi-dimensional effects of activating hair follicles, repairing scalp barriers and resisting scalp aging, greatly improving product competitiveness.
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