Syn ake powder serves as a standard sample for synthesizing tripeptide derivatives, used in comparative experiments related to peptide molecular structure modification and activity optimization, The core anti-wrinkle synthetic peptide raw material mimics the biomimetic snake venom activity mechanism, with a purity of ≥98%, is easily soluble in water, and can soothe dynamic wrinkles and reduce wrinkles and tighten the skin in the long term with a low addition of 0.01%. It is compatible with various anti-aging skin care formulas and is compliant and stable.providing a reference for the development of novel biomimetic peptide drugs. Kpeptide is committed to providing you with the best technical support and the most comprehensive after-sales support.
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Syn Ake Powder COA
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| Certificate of Analysis | ||
| Compound name | Dipeptide Diaminobutyroyl Benzylamide Diacetate/Syn Ake Powder | |
| Grade | Pharmaceutical grade | |
| CAS No. |
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| Quantity | 54g | |
| Packaging standard | PE bag+Al foil bag | |
| Manufacturer | Shaanxi BLOOM TECH Co., Ltd | |
| Lot No. | 202601090051 | |
| MFG | Jan 9th 2026 | |
| EXP | Jan 8th 2029 | |
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| Item | Enterprise standard | Analysis result |
| Appearance | White or almost white powder | Conformed |
| Water content | ≤5.0% | 0.37% |
| Loss on drying | ≤1.0% | 0.52% |
| Heavy Metals | Pb≤0.5ppm | N.D. |
| As≤0.5ppm | N.D. | |
| Hg≤0.5ppm | N.D. | |
| Cd≤0.5ppm | N.D. | |
| Purity (HPLC) | ≥99.0% | 99.52% |
| Single impurity | <0.8% | 0.37% |
| Total microbial count | ≤750cfu/g | 80 |
| E. Coli | ≤2MPN/g | N.D. |
| Salmonella | N.D. | N.D. |
| Ethanol (by GC) | ≤5000ppm | 452ppm |
| Storage | Store in a sealed, dark, and dry place below -20°C | |
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Precisely Targeted Binding Site Aspect
Specifically targets the ε-subunit of nmAChR
As a reversible antagonist, Syn ake powder selectively binds to the ε-subunit of the muscle nicotinic acetylcholine receptor, precisely occupying the natural binding site of endogenous acetylcholine and completely blocking the binding pathway between acetylcholine and the receptor. It will not cause non-specific interference to neuronal nicotinic acetylcholine receptors, with extremely high target selectivity.
Serves for subtype localization verification in scientific research
Kpeptide Leveraging its characteristic of only targeting the α1-ε subunit interface, Syn-Ake Powder can be used as a specific probe to accurately distinguish the distribution differences between muscle-type and neuronal nicotinic acetylcholine receptors in neurobiology experiments. It assists in locating the expression positions of different receptor subtypes in muscle tissues and eliminates experimental interference from other receptor subtypes.
Replaces natural snake venom toxins for safe experiments
It fully replicates the target binding properties of natural Waglerin I toxin. Without directly using highly toxic natural snake venom, researchers can carry out studies on the target binding kinetics and structure-activity relationship of snake venom active molecules, greatly reducing the safety risks of biological experiments.
Blocking Signal Transduction Pathways
Achieves reversible muscle relaxation for anti-wrinkle effects. After the binding site is blocked, the ion channel remains closed continuously, blocking Na⁺ influx. Muscle cells cannot complete depolarization, and the contraction pulses transmitted from nerves to muscles are completely inhibited. Facial expression muscles are relaxed, which rapidly fades dynamic wrinkles. It can reduce muscle contraction frequency by 82% within 1 minute, and the effect of reducing expression lines can reach 35% in 28 days.
Constructs a research model for neuromuscular signaling pathways
By precisely blocking the excitatory transmission link on the postsynaptic membrane,
it artificially interrupts the signal transmission at the neuromuscular junction in in vitro cell experiments This is used to study the upstream and downstream signal cascade mechanisms of this pathway,providing a standardized intervention tool for physiological and pathological research related to muscle spasm and abnormal muscle tone.
Establishes a quantitative efficacy evaluation system for anti-wrinkle raw materials
Taking the blocking efficiency of this signaling pathway as the core quantitative indicator, a standardized efficacy evaluation system for anti-aging peptide raw materials is established in reconstructed skin and in vitro muscle cell models. It provides traceable experimental data support for the anti-wrinkle efficacy claims of cosmetic formulations.
Syn-Ake Powder in Constructing Neuromuscular Signal Pathway Research Models
Constructing a gradient-controllable synaptic transmission regulation model
In in vitro neuromuscular co-culture systems, tissue slices or engineered muscle constructs, by adjusting the concentration and exposure duration of Syn-Ake Powder, researchers can precisely titrate the partial blockade degree of muscle nicotinic acetylcholine receptors, achieving a gradient downregulation of signal transmission strength at the neuromuscular junction. Without destroying the native structure of synapses, it can simulate different levels of impaired synaptic transmission, and is used to study synaptic plasticity, receptor desensitization effects and downstream compensatory signaling mechanisms.
Establishing a receptor function adaptation research model
By setting up Syn-Ake Powder intervention groups with different gradients to simulate the progressive reduction of receptor availability, it can be used to systematically observe how motor neurons and muscle fibers complete functional compensation by upregulating receptor subunit expression and regulating the activity of downstream signaling pathways,
providing a standardized intervention tool for analyzing the homeostasis maintenance mechanism of neuromuscular junctions.
Building a comparative research platform for receptor kinetics
Relying on its reversible binding property, in the same set of neuromuscular pathway models, the blocking kinetics and functional recovery processes of nicotinic acetylcholine receptors under different experimental conditions can be compared through the cyclic operation of drug administration and elution. This greatly reduces the interference of individual experimental differences on results and improves the repeatability of pathway mechanism research.
Realizing a differentiation model for subtype-specific receptor functions
Using the characteristic that Syn ake powder only specifically targets the α1-ε subunit interface of muscle-type nicotinic acetylcholine receptors, it can accurately distinguish the independent functions of muscle-type and neuronal nicotinic acetylcholine receptors in neuromuscular pathway models,
eliminate the signal interference from other receptor subtypes, clarify the exclusive roles of different receptor subtypes in neuromuscular signal transmission, and assist in completing the functional localization and signal specificity verification of receptor subtypes.
Constructing a pathological simulation model for neuromuscular diseases
Through the controllable partial blocking effect, it can simulate the phenotype of insufficient receptor function under pathological states such as congenital myasthenia syndrome and myasthenia gravis, and build a simplified pathological research model without gene editing. It provides a low-cost, highly reproducible experimental carrier for drug screening and mechanism exploration of related neuromuscular conduction disorders.
Syn-Ake Powder in Receptor Subtype Localization and Verification for Scientific Research
Highly selective subtype-specific labeling probe
Syn-Ake Powder exclusively targets the α1-ε subunit interface of muscle-type nicotinic acetylcholine receptors (mnAChR), with no non-specific binding to neuronal nAChR subtypes such as α7, α4β2 and α3β4. It can act as a highly specific competitive probe to precisely distinguish mnAChR from other nAChR subtypes in complex biological samples, eliminating cross-interference from non-target receptors during localization experiments.
Spatial distribution mapping of mnAChR in tissues
By coupling Syn-Ake Powder with fluorescent tags or isotope tracers, Kpeptide researchers can perform in-situ localization imaging of mnAChR in muscle tissue slices, neuromuscular junction specimens and engineered muscle constructs. This helps directly visualize the spatial distribution, density and clustering status of muscle-type nAChRs on the postsynaptic membrane, providing intuitive experimental evidence for studying the assembly pattern of neuromuscular junctions.
Verification of subtype-specific binding selectivity
In recombinant cell lines that separately express different nAChR subtypes, Syn-Ake Powder can be used as a reference antagonist to verify the subtype selectivity of newly synthesized nAChR-targeted peptides or small-molecule compounds. Its well-documented exclusive affinity for mnAChR serves as a gold standard control to confirm whether the test compound has specific targeting properties for the muscle receptor subtype.
Functional validation of subtype gene knockout/knockin models
In genetically modified animal or cell models with nAChR subunit knockout or point mutation, Syn-Ake Powder can be used to detect the changes in receptor binding affinity and functional response. This directly verifies whether the target subtype gene modification successfully alters the normal expression and functional conformation of mnAChR, providing a functional verification indicator for the construction of neuromuscular research models.
Differential identification of fetal and adult mnAChR subtypes
Fetal muscle nAChRs contain the γ subunit instead of the ε subunit, which is the unique binding target of Syn Ake Powder. This characteristic can be used to accurately distinguish and quantify the expression ratio of adult-type (ε-containing) and fetal-type (γ-containing) nAChR subtypes in muscle tissues, supporting research on muscle development, post-injury muscle regeneration and related myopathy mechanisms.
Protocol for Rapid Differentiation Between Cells Expressing Muscle-Type α1β1δε nAChR and Cells Expressing Neuronal nAChR Subtypes
Electrophysiological Functional Screening
Pre-treat both groups of cells with 10 μM Syn-Ake Powder for 5 minutes, then apply acetylcholine to activate the receptor channels. For cells that only express the muscle-type α1β1δε subtype, the inward current evoked by acetylcholine will be completely blocked, the ion channels cannot open, and no obvious current response can be recorded. For cells expressing neuronal subtypes, the receptor channel activity remains completely unaffected. Stable characteristic inward currents can still be detected after acetylcholine stimulation, and the distinction can be finished within 10 minutes based on the difference in current responses.
Fluorescent Probe Labeling Method
Kpeptide Conjugate Syn ake powder with a fluorophore (such as FITC) to prepare a subtype-specific fluorescent probe.
Incubate the mixed cell sample with this probe for 30 minutes, then observe via flow cytometry or fluorescence microscopy: cells expressing the muscle-type α1β1δε subtype will emit specific fluorescent signals, while cells expressing neuronal subtypes show no fluorescent labeling, enabling direct sorting and differentiation.
Ligand Competitive Binding Assay
In a 96-well plate, add isotope-labeled α1β1δε subtype-specific tracers and gradient concentrations of Syn-Ake Powder to the mixed cell samples simultaneously.
In cells that only express the muscle-type α1β1δε subtype, Syn-Ake Powder will competitively occupy the receptor binding sites, leading to a gradient decrease in the tracer binding rate as the drug concentration rises. In cells expressing neuronal subtypes, the tracer binding rate remains completely unaffected. The difference in binding rates allows rapid batch differentiation of subtype-specific cells.
Reference
1.Function of dipeptide diaminobutyroyl benzylamide diacetate in skin.
2.The effect of a serum containing acetyl hexapeptide-8, dipeptide diaminobutyroyl benzylamide diacetate and gluconolactone on skin biomarkers.
FAQ
Q: What is the exact purity and batch stability of this product?
A: The purity of our Syn-Ake Powder is stably above 98%, each batch comes with an official COA test report, and the product maintains stable properties under normal storage conditions for 24 months.
Q: What are your available package sizes and corresponding prices? Do you support small trial orders for R&D use?
A: We offer multiple specifications from 100mg trial packs to 100kg bulk industrial orders. The 100mg trial pack is priced at USD 225, and we support small R&D trial orders with fast delivery.
Q: How long can I receive the goods after placing the order? Do you support international DDP door-to-door delivery?
A: Spot orders can be shipped within 48 hours, and we support international DDP door-to-door delivery services to most countries and regions, with a standard delivery cycle of 3-7 working days.
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