Syn ake cream core ingredient is a synthetically produced snake venom-like tripeptide-dipeptide diaminobutyryl benzylamide diacetate. It perfectly mimics the activity mechanism of Waglerin I toxin in Tropidolaemus wagleri venom. It reversibly blocks the transmission of nerve signals to muscles, gently inhibiting dynamic facial muscle contractions. Combined with betaine, natural plant extracts, and hyaluronic acid, it offers both anti-wrinkle and moisturizing benefits. Kpeptide has a variety of protein expression systems that can achieve protein expression at different scales according to customer needs.
Our products
Syn Ake Cream COA
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| Certificate of Analysis | ||
| Compound name | Dipeptide Diaminobutyroyl Benzylamide Diacetate/Syn Ake Cream | |
| Grade | Pharmaceutical grade | |
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| Quantity | 72g | |
| Packaging standard | PE bag+Al foil bag | |
| Manufacturer | Shaanxi BLOOM TECH Co., Ltd | |
| Lot No. | 202601090051 | |
| MFG | Jan 9th 2026 | |
| EXP | Jan 8th 2029 | |
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| Item | Enterprise standard | Analysis result |
| Appearance | White or almost white powder | Conformed |
| Water content | ≤5.0% | 0.66% |
| Loss on drying | ≤1.0% | 0.72% |
| Heavy Metals | Pb≤0.5ppm | N.D. |
| As≤0.5ppm | N.D. | |
| Hg≤0.5ppm | N.D. | |
| Cd≤0.5ppm | N.D. | |
| Purity (HPLC) | ≥99.0% | 99.66% |
| Single impurity | <0.8% | 0.42% |
| Total microbial count | ≤750cfu/g | 80 |
| E. Coli | ≤2MPN/g | N.D. |
| Salmonella | N.D. | N.D. |
| Ethanol (by GC) | ≤5000ppm | 477ppm |
| Storage | Store in a sealed, dark, and dry place below -20°C | |
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Signal Pathway Blocking & Neurobiology Research
Kpeptide With its core active ingredient Dipeptide Diaminobutyroyl Benzylamide Diacetate, Syn ake cream selectively binds to the ε-subunit of muscular nicotinic acetylcholine receptors (nmAChR), competitively blocks the binding between acetylcholine and its receptor, inhibits post-synaptic sodium ion influx and depolarization, and achieves reversible neuromuscular signal conduction blockade. It serves as a valuable research tool in multiple neurobiology scenarios:
Signal Transduction Pathway Research
Neuromuscular Junction Mechanism Study: As a highly selective nmAChR ε-subunit antagonist, it can specifically block acetylcholine-mediated excitatory conduction at the post-synaptic membrane, helping researchers precisely isolate the independent role of this receptor subtype in muscle contraction signaling pathways without interference from other nicotinic acetylcholine receptor subtypes. Reversible Signal Blocking Model Construction: Different from irreversible neurotoxin tools, the blocking effect of Syn Ake Cream fully returns to baseline after 4 days,
enabling the construction of a time-controllable "temporary nerve signal block - functional recovery" experimental model to study muscle function reconstruction mechanisms after signal pathway reactivation. Sodium Channel Associated Pathway Verification: By blocking post-synaptic Na⁺ uptake and depolarization, it acts as a probe molecule to verify the activation logic of downstream linked signaling steps such as action potential generation and muscle contraction coupling, assisting in parsing the complete conduction chain of neuroexcitation-contraction coupling.
Neurobiology Research Tool Applications
Standard Reagent for Muscle Cell Contraction Regulation Experiments: At a concentration of 0.05mM, it reduces muscle contraction frequency by 82% in vitro myotube contraction assays, serving as a standardized tool for activity comparison and efficacy gradient calibration of candidate myorelaxant compounds.
In Vitro Model Construction for Neurodevelopmental Disorders: It can be used to build in vitro cell models of pathological states such as temporary muscle spasm and excessive muscle tone,
assisting in the screening of candidate intervention molecules for neurological diseases characterized by overexcited nerve signals.
Specific Applications in Reversible Signal Blocking Model Construction
Core Mechanism Basis for Reversible Model Construction
Reversible Receptor Binding: Unlike permanent neuroblocking agents, Syn-Ake binds to the ε-subunit of muscle nicotinic acetylcholine receptors (nmAChR) in a fully reversible manner. Once topical application stops, the active ingredient gradually dissociates from the receptors, and blocked nerve signal conduction will automatically return to the normal baseline level after 4 days without causing permanent damage to neuromuscular structures. Localized Non-Systemic Action: When applied topically, Syn Ake Cream only acts on the neuromuscular junction under the treatment site, with no systemic distribution. This feature allows researchers to set an untreated control area on the same experimental subject, eliminating individual differences between different groups. Verified Quantifiable Efficacy: In vitro muscle cell contraction assays confirm that 0.05 mM Syn-Ake can reduce muscle contraction frequency by 82% after 2 hours, the inhibitory effect remains 67% lower after 2 days, and the function fully recovers on the 4th day. This stable, dose-dependent efficacy provides a standardized quantitative reference for model construction.
Typical Specific Research Use Cases
Time-Window Controlled Nerve Function Recovery Model: Kpeptide Researchers can set different administration cycles to construct gradient reversible blocking models of 2h, 2d and 4d, to track the dynamic changes of downstream gene expression, ion channel activity and muscle fiber morphology during the whole process from signal blocking to full functional recovery. Myorelaxant Drug Efficacy Evaluation Positive Control Model: As a standardized reversible positive blocking system, it can be used to compare the difference in signal regulation efficiency between new candidate myorelaxant compounds and Syn-Ake, and quickly screen lead molecules with better duration and safety. Dynamic Expression Line Intervention Preclinical Model: The topical reversible blocking feature can simulate the clinical effect of temporary neuromodulation on facial expression muscles, which is used to evaluate the long-term skin adaptability and wrinkle improvement mechanism of anti-wrinkle formulations, avoiding the irreversible muscle stiffness risk brought by botulinum toxin intervention models.
Neuromuscular Junction Signal Transduction Teaching Demonstration Model: With its low biotoxicity and fully reversible effect, it can be used as a safe experimental teaching tool to intuitively demonstrate the complete process of "acetylcholine binding - signal blocking - function recovery" at the neuromuscular junction for neurobiology courses.
Core Demonstration Logic of Signal Transduction Process
It clearly visualizes every key node of the classic neuromuscular signal transmission chain:
First, the normal baseline state is demonstrated: when action potential arrives at the motor nerve terminal, acetylcholine (ACh) is released into the synaptic gap, ACh binds to nicotinic acetylcholine receptors (nAChR) on the postsynaptic membrane, triggering Na⁺ influx, postsynaptic depolarization and final muscle contraction.
After Syn ake cream is applied, the blocking process is intuitively presented: its core tripeptide component competitively occupies the ε-subunit of muscle nAChR, prevents endogenous ACh from binding to the receptor, interrupts the subsequent ion influx and depolarization process, and finally inhibits muscle contraction, helping students quickly grasp the central role of postsynaptic receptors in signal transduction.
Unique Teaching Advantages That Traditional Reagents Cannot Achieve
Full reversible dynamic demonstration: Different from irreversible neurotoxins such as botulinum toxin, the blocking effect of Syn Ake Cream can be completely eliminated after repeated elution with normal tissue fluid. The whole process of "normal contraction →
partial contraction inhibition → complete signal block → full functional recovery" can be completed within 30-40 minutes in one class, allowing students to directly distinguish the essential difference between reversible antagonists and irreversible nerve blocking agents. Extremely high teaching safety: It has no strong lethality or sensitization of natural snake venom toxins, no special biosafety protection requirements, and can be operated directly in ordinary undergraduate physiology teaching laboratories without additional safety risks. Quantitative dose-effect demonstration: By preparing Syn Ake Cream working solutions of different concentration gradients, students can clearly observe that the muscle contraction tension decreases gradually with the increase of drug concentration under the same electrical stimulation parameters, which intuitively demonstrates the basic pharmacological law of dose-dependent signal regulation.
Standard Teaching Demonstration Operation Implementation
The whole demonstration can be completed based on the classic isolated sciatic nerve-gastrocnemius specimen,
Add low-concentration Syn Ake Cream working solution to the specimen bath, incubate for 3-5 minutes, and students can observe that the muscle contraction amplitude drops significantly after the same stimulation, showing the state of partial signal block.
Increase the drug concentration appropriately, and the muscle contraction will be almost completely suppressed, presenting the state of full signal transduction interruption.
Rinse the specimen 3-4 times with fresh Ringer's solution, wait for 10-15 minutes and re-stimulate, the muscle contraction tension will gradually return to the level close to the initial baseline, completing the full dynamic demonstration of the entire neuromuscular signal transduction process.
Vitro Myotube Reversible Contraction Regulation Models
Standardized Construction of Reversible Contraction Phenotype
It can rapidly induce stable, dose-dependent contraction inhibition in differentiated C2C12 or primary human myotubes. At the optimized working concentration of 0.05 mM, it can reduce myotube contraction frequency by up to 82% within 2 hours. After simple medium elution, the myotube contraction function can fully recover to the baseline level within 4 days, helping researchers build a highly reproducible "contraction inhibition - functional recovery" reversible phenotype without causing permanent cytotoxic damage.
Pharmacological Evaluation Platform for Myorelaxant Candidate Compounds
It serves as a validated positive control system for high-throughput drug screening. Kpeptide Researchers can pre-establish a stable reversible contraction inhibition baseline via Syn Ake Cream, then test the reversal efficiency of different candidate myorelaxant or antispasmodic molecules on the blocked signal pathway. This system effectively reduces inter-sample individual differences and significantly improves the stability and comparability of efficacy data.
Mechanism Study of Excitation-Contraction Coupling Pathway
By precisely controlling the "blocking-recovery" time window of Syn Ake Cream,
Kpeptide researchers can dynamically track the sequential changes of downstream ion channel activity, calcium transient response, myofibril arrangement and related gene expression profiles during the whole process from signal interruption to functional restoration. This helps to further reveal the regulatory mechanism of the neuromuscular excitation-contraction coupling axis.
Simulation of Pathological Models of Muscle Hypercontraction
It can be used to construct an in vitro simulation system for myospasm, excessive muscle tone and other related pathological states. By adjusting the duration and intensity of Syn ake cream intervention, researchers can quantitatively analyze the damage of long-term abnormal contraction to myotube structure and metabolic function, and provide a more physiologically relevant in vitro evaluation model for the development of intervention drugs for muscle hypertonia diseases.
Reference
1.Function of dipeptide diaminobutyroyl benzylamide diacetate in skin.
2.The effect of a serum containing acetyl hexapeptide-8, dipeptide diaminobutyroyl benzylamide diacetate and gluconolactone on skin biomarkers.
FAQ
Q: What is the maximum reversible blocking duration of Syn Ake Cream in in vitro myotube contraction models? Will it cause permanent damage to C2C12 differentiated myotubes?
A: The maximum duration of effective signal blocking can last up to 48 hours under standard working concentration. Multiple independent cytotoxicity assays have confirmed that Syn Ake Cream will not induce permanent cell apoptosis or irreversible structural damage to myotubes within the effective working concentration range, and all neuromuscular signal functions can fully return to baseline after 4 days of elution.
Q: Can Syn Ake Cream be used to build a reversible signal blocking model on isolated neuromuscular specimens, or is it only suitable for cell-level experiments?
A: It works for both scenarios. It can not only be applied in in vitro myotube cell systems, but also used in isolated sciatic nerve-gastrocnemius specimens, 3D bioprinted engineered muscle tissues and other tissue-level experimental systems, with highly consistent reversible blocking performance.
Q: Why is Syn Ake Cream more suitable for building reversible signal models than traditional nicotinic acetylcholine receptor antagonists such as α-bungarotoxin?
A: Unlike irreversible natural neurotoxins, Syn Ake Cream has no permanent binding effect on receptors, requires no special biosafety level-2 lab protection, and its blocking effect can be completely eliminated through simple medium elution, which significantly improves experiment safety and result reproducibility.
Q: Will Syn Ake Cream interfere with downstream Western blot or qPCR testing of myotube samples?
A: No. The peptide component of Syn Ake Cream does not interact with common protein extraction reagents or nucleic acid amplification systems, and will not produce non-specific bands or interfere with gene expression quantification results.
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