Walnut oligopeptides are a series of highly bioactive small molecule fragments extracted from Juglans regia L. (walnut kernel) via advanced controlled enzymatic hydrolysis technology. The molecular weight of our WOPs is predominantly below 500 Daltons. This low-molecular-weight characteristic enables fast dissolution and excellent bioavailability. The finished product system contains a mixture of free amino acids, functional oligopeptides, small peptides, and trace residual proteins, retaining the natural nutritional spectrum of walnut raw materials.
Kpeptide walnut oligopeptides are rich in 18 types of amino acids, covering all 8 essential amino acids that the human body cannot synthesize independently. Among the amino acid profile, glutamic acid ranks first in content, followed by arginine. Glutamic acid is a core participant in human nitrogen metabolism.
Walnut Oligopeptide COA
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| Certificate of Analysis | ||
| Compound name | Walnut Oligopeptide | |
| Grade | Pharmaceutical grade | |
| Quantity | 20g | |
| Packaging standard | PE bag+Al foil bag | |
| Manufacturer | Shaanxi BLOOM TECH Co., Ltd | |
| Lot No. | 202601090066 | |
| MFG | Jan 9th 2026 | |
| EXP | Jan 8th 2029 | |
| Structure | N/A | |
| Item | Enterprise standard | Analysis result |
| Appearance | White or almost white powder | Conformed |
| Water content | ≤5.0% | 0.40% |
| Loss on drying | ≤1.0% | 0.21% |
| Heavy Metals | Pb≤0.5ppm | N.D. |
| As≤0.5ppm | N.D. | |
| Hg≤0.5ppm | N.D. | |
| Cd≤0.5ppm | N.D. | |
| Purity (HPLC) | ≥99.0% | 99.98% |
| Single impurity | <0.8% | 0.42% |
| Total microbial count | ≤750cfu/g | 205 |
| E. Coli | ≤2MPN/g | N.D. |
| Salmonella | N.D. | N.D. |
| Ethanol (by GC) | ≤5000ppm | 520ppm |
| Storage | Store in a sealed, dark, and dry place below 2-8°C | |
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Application
Walnut oligopeptides, the biologically active essence extracted from the "brain nourishing fruit" of walnuts, are becoming one of the most promising star raw materials in functional food and general health industry with its six core effects of neuroprotection, cardiovascular protection, immune regulation, intestinal maintenance, skin anti-aging, anti fatigue and anti-oxidation. Brain health and neuroprotection - the most dazzling star effect The performance of walnut oligopeptides in the field of brain health is truly stunning.
(1) Improve learning and memory abilities
An experiment on juvenile mice (48 male C57BL/6J mice) showed that the mice were randomly divided into a blank control group and three walnut peptide intervention groups: low (110mg/kg · bw), medium (220mg/kg · bw), and high (440mg/kg · bw). After continuous intervention for 30 days, Morris water maze, jumping platform experiment, and shuttle box experiment were conducted. The results show: Morris water maze:
The escape latency of the high-dose group was significantly shortened compared to the control group (P<0.05), and the residence time and number of passes in the target quadrant were significantly increased in the medium and high-dose groups (P<0.05); Jumping Platform Experiment: The low and high dose groups showed a decrease in the number of errors during the 24-hour test (P<0.05), while all three intervention groups had an increase in latency (P<0.05). The medium dose group had a slower memory loss of electric shocks (P<0.05); Shuttle box experiment: The low, medium, and high dose groups showed an increase in the number of active avoidance attempts (P<0.05) and a decrease in the number of electric shocks received (P<0.05). The conclusion is clear: it can significantly enhance the learning and spatial memory ability, active and passive avoidance ability, and may delay the decline of learning and memory in young mice. The mechanism is closely related to antioxidant activity - the walnut peptide dose group has higher serum T-SOD and GSH Px activities than the control group,
and significantly lower MDA levels than the control group (P<0.05). In addition, a study published in Food&Function successfully isolated a novel oligopeptide AQ from the hydrolysate of defatted walnut meal, and identified its chemical structure as AASDCQ. This peptide exhibits excellent free radical scavenging ability at a concentration of 1mg/mL: O ₂⁻ free radical scavenging rate of 81.00%, DPPH free radical scavenging rate of 79.40%, and ABTS free radical scavenging rate of 67.09%.
More importantly, AQ has a strong protective effect against H-SY5Y neuronal damage induced by H ₂ O ₂, significantly reducing cell damage and apoptosis. In vivo experiments have also shown that AQ can promote angiogenesis in quail chorioallantoic membrane and reduce ROS accumulation and prolong lifespan in the C. elegans model. Another clinical study has shown that it can improve age-related learning and memory disorders, and is considered a potential and effective intervention.
(2) Deep mechanisms of neuroprotection
AQ oligopeptides also exhibit potent anti-inflammatory and neuroprotective effects: in LPS stimulated RAW264.7 cells, AQ effectively inhibits the secretion of pro-inflammatory factor NO (IC ₅₀=46.03 ± 0.32 μ M) and suppresses the expression of IL-6 and TNF - α. Western Blot further confirmed its anti-inflammatory mechanism. Overall, AQ possesses four layers of neuroprotective properties: antioxidant, anti-inflammatory, pro angiogenic, and anti-aging. Information source: Food&Function (RSC Publishing)
2.Cardiovascular health - the natural shield that protects the heart
The protective effect of walnut oligopeptides on the cardiovascular system has been validated by multiple sources. Its mechanism of action includes:
Reduce levels of "bad" cholesterol (LDL);Regulating blood pressure; Improve blood circulation. These benefits stem from the synergistic effect of healthy fats, antioxidants, and essential amino acids in WOPs. In the context of increasingly severe global cardiovascular disease, this natural heart protection solution has significant public health implications.
3.Immune regulation - activating the human defense corps
Research has shown that it can significantly improve humoral immunity and cell-mediated immune responses, manifested as: Enhance the activity of natural killer cells (NK cells). This means that it is not only a "nutritional supplement", but also an immune modulator that can activate the innate immune system of the human body in all aspects.
4. Gut Health - Probiotic like "Nourishing Bacteria" Effect
WOPs have prebiotic properties and can serve as a nutritional source for beneficial gut microbiota, promoting the growth and reproduction of "friendly microorganisms" such as bifidobacteria and lactobacilli. The study also found that WOPs can promote bacterial growth and reproduction, increase bacterial biofilm and extracellular polysaccharide (EPS) secretion. By maintaining a healthy gut microbiota ecology, it indirectly enhances digestive function and nutrient absorption efficiency.
4.Skin Health - The Anti Aging Code from Inside Out
In the field of cosmetics, walnut oligopeptides also shine brightly: Stimulating the proliferation of keratinocytes: These cells are responsible for producing keratin, the core component of the skin's protective barrier, which promotes skin repair and regeneration; Promote the synthesis of collagen and elastin: These two proteins are key to maintaining skin elasticity and firmness, and can effectively combat skin sagging and aging; Dual protection of antioxidant and anti-inflammatory: reducing the damage of free radicals to the skin and delaying chronic aging.
Skin Care
6. Anti fatigue and comprehensive antioxidant
It has also been proven to have anti fatigue ability and strong antioxidant capacity. Researchers have found that it can eliminate hydroxyl radicals and superoxide anion radicals, which is the molecular basis for its various health benefits.
Information source: Shaanxi Shanhe Biotechnology Product Description (2026-01-29)
Manufacturing information
The preparation of WOPs mainly adopts multi-step processes such as enzymolysis - ultrafiltration - gel filtration chromatography. Specifically, the defatted walnut meal was first used as raw material, enzymatically hydrolyzed by protease, then the components with different molecular weights were separated by ultrafiltration membrane, and finally the target oligopeptides were purified by gel filtration chromatography.
Taking the preparation method of the test solution described in patent WO2017124921A1 as an example: Accurately weigh 10mg of WOP powder, place it in a 50mL brown volumetric flask, add 30mL of ethanol, sonicate for 5 minutes, dilute with ethanol to the mark, and shake well. This indicates that WOP powder has good ethanol solubility.
In terms of product form, the market is mainly divided into four types: liquid type, dry powder type, peptide type, and oligopeptide type. suitable for food formulas and dietary supplements. It appears as a fine white to milky yellow powder, without clumping or impurities, with a purity of over 95% or even 99%.
Shipping Process of Walnut Oligopeptides
Pre-Shipping Preparation & Quality Inspection
Before shipment, all Kpeptide WOPs products will undergo strict factory quality inspection by our professional QC team. The inspection items include product purity, amino acid content stability, molecular weight distribution, moisture content, microbial indicators and packaging sealing performance, to ensure that each batch of products complies with international functional food and raw material export standards. Unqualified products will be rejected and reprocessed, and only products with complete test reports and qualified indicators can enter the shipping link. Meanwhile, we sort and count products according to customer order requirements, confirm product specifications, quantity, batch number and delivery address, and complete the sorting and grouping of goods to ensure order accuracy.
Professional Moisture-Proof & Shock-Proof Packaging Treatment
In view of the characteristics that walnut oligopeptide powder is easy to absorb moisture, agglomerate and be affected by external extrusion, Kpeptide adopts multi-layer professional packaging for all WOPs products.
The inner layer uses food-grade high-barrier aluminum foil vacuum packaging, which effectively isolates air, moisture and dust, locks the biological activity of oligopeptides and amino acid nutrients, and prevents product oxidation and deterioration. The middle layer is filled with shock-absorbing bubble cotton and foam pads for all-round buffering and anti-collision protection. The outer layer adopts thickened corrugated cartons or export-standard wooden boxes according to the order volume and transportation mode, which can resist extrusion and friction during long-distance transportation. All packaging boxes are marked with clear product information, batch number, production date, shelf life and international shipping marks such as "moisture-proof", "shock-proof" and "upward" to standardize handling operations.
Standard Warehousing Handover & Loading Management
After packaging is completed, qualified Kpeptide WOPs products will be transferred to a constant-temperature and dry professional export warehouse for temporary storage. The warehouse maintains a constant temperature of 15-25°C and a humidity below 60%,
avoiding high temperature, high humidity and direct sunlight that may affect product stability. During the loading process, our special staff supervise the whole process, standardize the stacking and placement of goods, avoid overstocking and heavy pressure, and classify and place products of different specifications to prevent confusion. For container transportation, we carry out fixed reinforcement inside the container to prevent goods from shaking, colliding and tipping during transportation. All loading operations comply with international cargo transportation specifications to ensure neat and stable cargo stacking.
Whole-Process Controlled Transportation & Environmental Monitoring
Kpeptide Walnut oligopeptides selects formal and qualified international logistics and freight forwarding companies for product transportation, and supports multiple transportation modes including sea freight, air freight and land freight according to customer needs and delivery cycle requirements. During the entire transportation process, we implement real-time dynamic monitoring of the logistics process. For long-distance cross-border transportation,
we equip temperature and humidity monitoring equipment in the transport vehicles and containers to track and record the storage environment of the products in real time, ensuring that the products are always in a dry, constant-temperature and stable transportation environment. Our logistics team tracks the cargo transportation status 24 hours a day, timely masters the departure, transit, customs clearance and arrival progress of the goods, and promptly feeds back the transportation information to customers. In addition, all exported Kpeptide WOPs products are equipped with complete customs declaration documents, quality inspection certificates and product qualification certificates to ensure smooth customs clearance and compliant transportation.
Post-Arrival Confirmation & After-Sales Guarantee
After the goods arrive at the destination port or warehouse, we actively cooperate with customers to complete cargo inspection and acceptance. If there are problems such as packaging damage, goods shortage or abnormal product quality caused by transportation, our after-sales team will respond quickly,
verify the situation and provide targeted solutions such as replacement and compensation. We always take product transportation safety and customer rights and interests as the core, and ensure that every batch of Kpeptide Walnut oligopeptides delivered to customers maintains stable quality and complete biological activity.
Reference
1.European Walnut Protein Peptide Market Report (2026-2033);
2.Shaanxi Shanhe Biotechnology Product Description
3.Food&Function (RSC Publishing) - Neuroprotective Study of a Novel Oligopeptide AQ
4.Journal of Peptide Nutrition and Health - Learning and Memory Experiment in Young Mice
5.PMC (T Ding, 2021) - Probiotic effects of walnut oligopeptides on gut microbiota
WO2017124921A1/MY193780A - Patent Document
6.Shaanxi ShineHerb Biological (ShineHerb) Product Page (2026-01-29)
7.Shaanxi Baichuan Biotechnology Official Website (September 9, 2025)
8.European Walnut Protein Peptide Market Report (2026-2033, CAGR 14.9%)
9.Global Walnut Peptide Industry Report (2026-2033)
10.Morgana's Alchemy Skin Care - Walnut Extract Skincare Benefits
11.Research on Walnut Oligopeptide Delayed Improved Aging Related Learning
12.Immunomodulatory effects of walnut oligopeptides research
Frequently Asked Questions
Q: What amino acids does Kpeptide WOPs contain?
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A: Kpeptide WOPs contains 18 kinds of natural amino acids, covering all 8 essential amino acids that the human body cannot synthesize independently. Among them, glutamic acid occupies the highest content, followed by arginine, which endows the product with unique nutritional advantages for brain nutrition and human metabolic regulation.
Q: What is the core advantage of glutamic acid in WOPs?
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A: Glutamic acid is a key substance in human nitrogen metabolism and the only amino acid that can be directly oxidized and utilized by brain tissue. It can serve as a direct energy source for brain cells, effectively supporting brain metabolism, relieving mental fatigue and maintaining normal mental function.
Q: What are the main functional effects of Kpeptide WOPs?
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A: Relying on its unique amino acid formula and oligopeptide activity, Kpeptide WOPs mainly supports brain energy metabolism, improves mental vitality, assists human nitrogen balance regulation, and supplements high-quality plant protein and amino acid nutrients. It is a natural functional raw material for brain-nourishing and nutritional supplementation.
Q: Is WOPs easy to cause allergies?
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A: Our WOPs adopts precise enzymatic hydrolysis technology to decompose macromolecular allergen proteins. The product has low allergenicity, pure ingredients and no added irritants. It is mild and safe for most people to take and apply.
Q: What are the storage requirements for Kpeptide WOPs?
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A: Please store the product in acool, dry, ventilated and dark environment, with ambient temperature controlled at 15–25°C and humidity below 60%. Seal the package tightly after opening to avoid moisture absorption, agglomeration and oxidation inactivation. Do not store in high temperature, humid or direct sunlight environments.
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